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Bascom Palmer Eye Institute

Calendar of Events

Bascom Palmer Eye Institute hosts a number of lectures and seminars, open to ophthalmologists, vision researchers, the University of Miami community and, in some instances, the general public. For a complete schedule of Continuing Medical Education events for the 2013-14 academic year, you may download the brochure with registration forms. If you wish to obtain additional information about any of these events please contact the respective coordinator.

McKnight Vision Speial Research Seminar- Presenter: Hyungsik Lim, PhD, Asst. Professor, Hunter College of the City University of New York

Calendar: McKnight Vision Research Seminars

“Imaging Microtubules in the Retinal Ganglion Cells by Second-Harmonic Generation”

Imaging Microtubules in the Retinal Ganglion Cells by Second-Harmonic Generation Here we will describe a novel label-free technique for visualizing the retinal nerve fibers, namely second-harmonic generation (SHG) microscopy, which allows us to measure the structural integrity of microtubules in the axons of the retinal ganglion cells. The cytoskeletal element has been suggested to be involved in the pathogenesis of glaucoma, thus may facilitate early detection of the disease. The imaging method has never been explored for the purpose of visualization of the retinal nerve fibers, so we will first explain how the intrinsic signal of SHG arises from intact microtubules and discuss the properties of this nonlinear optical radiation as relevant for glaucoma. Using the technique, we have investigated the dynamics of microtubule network in the retina in ways that have not been possible. In this talk, we will show how SHG imaging enables studies of glaucomatous events. We will present the results of our recent experiments where we examined alterations of the axonal microtubules in the physiological as well as pathological retinas. Finally, we will discuss the prospects of the new imaging technique for longitudinal studies of in vivo animals, screening of effective therapeutics, and ultimately human diagnostics.