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Bascom Palmer Eye Institute

Calendar of Events

Bascom Palmer Eye Institute hosts a number of lectures and seminars, open to ophthalmologists, vision researchers, the University of Miami community and, in some instances, the general public. For a complete schedule of Continuing Medical Education events for the 2013-14 academic year, you may download the brochure with registration forms. If you wish to obtain additional information about any of these events please contact the respective coordinator.

McKnight Vision Research Seminar- Presenter: Dr. Vladlen Slepak, Professor- November 13th

Calendar: McKnight Vision Research Seminars

“Expression of olfactory system genes in the eye”
To advance our understanding how the outer eye interacts with its environment, we asked which cellular receptors are expressed in the cornea, focusing on G protein-coupled receptors (GPCRs). We subjected total RNA from the mouse cornea to next-generation sequencing, validating the data by RT-PCR and a novel high-resolution in situ RNA hybridization technology, RNAscope. NGS identified more than 150 GPCR transcripts, of which more than 100 were putative olfactory receptors (Olfr’s). RT-PCR analysis and RNAscope confirmed the presence of related genes, including olfactory marker protein (OMP) and the G protein associated with olfaction, Golf. In addition to cornea, one of Olfrs, Olfr558, was highly expressed in vessels of the eye that co-stained with antibodies against alpha-smooth muscle actin, indicating expression in arterioles. Thus, several species of mRNA encoding putative olfactory receptors and related genes are expressed in the eye indicating that they may play a role in detection of chemicals on the ocular surface or play a role in regulation of ocular blood flow.