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Bascom Palmer Eye Institute

Analytic Imaging

The Analytic Imaging Facility supports the care and use of instrumentation for scanning laser confocal microscopy and phosphorimaging. It also provides software, computers, and technical expertise for manipulation and analysis of photographic and digital images, and quantification of image features.

Leadership

The Analytic Imaging Facility is co-directed by:

Valery I. Shestopalov, PhD
Associate Professor of Ophthalmology

Pedro I.J. Salas, PhD
Associate Professor of Cell Biology & Anatomy

Location

The Analytic Imaging Facility is located on the 7th floor of the McKnight building, room 704a and 704b.

Equipment

  • Leica TCS SP5 Confocal Microscopy Instrumentation

Click here for availability calendar

  • NIH grant S10 RR019382 funded the purchase of this instrumentation.

The Leica TCS SP5 system is capable of multiple excitation wavelengths of 405, 458, 476, 488, 496, 514, 543 and 633 nm. Besides the detection system for 4 fluorescence channels and one transmission channel simultaneously, this system is also equipped with an acusto-optical-beam-splitter which provides the highest flexibility for setting up the required experimental parameters. The scope is equipped with 5x, 10x, 20x, 40x, and 63x objectives. System allows great user flexibility with resolution (up to 64 megapixels) and frame rate (up to 200fps).

Both inverted and upright microscopes are available. The inverted scope is surrounded with an acrylic chamber enclosure to control environmental factors for live cell imaging.

  • Dell Computer with 3.0 ghz Xeon processor, 3 gig RAM, and software

This computer and the Velocity software described below was purchase with an earmarked gift from Estelle and George Rosenfield.

  • Volocity 3D Rendering Software (Improvision Inc., Lexington, MA

This software package consists of two parts – Volocity Visualization (rendering part) and Volocity Classification (3D/4D image analysis part). This product provides excellent visualization and editing tools along with great measurement and “feature search” capabilities. It allows you to discover, visualize and measure features hidden inside cells or tissues via analysis of images after restoration and rendering.

The Volocity 3D Rendering software is the first true color 4D rendering software (3D plus time) designed for biomedical imaging. It uses new, highly advanced algorithms to provide high-speed easy to use, interactive rendering of time resolved color 3D volumes. This software allows volumetric object measurements, quantitative analysis of 4D images and compiling such images into movies.

  • Leica Application Suite

Leica application suite is useful for processing confocal images. It is capable of exporting image/movie files, photo manipulation (cropping, contrast adjustments, background subtraction), 3d projection/3d deconvolution, intensity measurements, colocalization measurements, and area measurements.

  • HP LaserJet 3700dtn

This printer provides for publication quality color prints of images.

  • Molecular Dynamics Phosphorimager

An end of year NIH grant administrative supplement to R01 EY012651-S1 funded the purchase of this instrumentation.

The Typhoon 9410 Trio+ phosphorimager is a three-laser base instrument, which is upgradable to 4-laser version given the need in additional blue line excitation will arise in the future. This configuration will work for radioactivity- and two-color fluorescence-labeled tissues, 1D and 2D gels, plates and arrays. The Typhoon is equipped with ImageQuant Image Analysis Software and is optimized with Ettan DIGE and DeCyder Differential Analysis Software for full automatization of proteomics analysis using 2D gels. It can detect multiple types of labeling: fluorescence, chemiluminescence and radioactivity.

This instrument has more than 30 different applications including Western, Northern, and Southern blots, primer extension and RNAse protection experiments, two-color and one-color microarrays, and 1D and 2D multiple-labeled gels. The Typhoon 9410 is preset for the analysis of multiple –labeled protein 2D gels for differential high-throughput screens.