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Bascom Palmer Eye Institute

Time-Lapse Microscopy

The Time-Lapse Microscopy Facility supports the care and use of the Carl Zeiss Axiovert 200m and accessories. This instrumentation provides the capabilities for time-lapse imaging of live cell dynamics. It also provides multi-fluorescence modalities such as quantitative calcium imaging and FRET, and has software add-ons for cell/object counting and mosaic image acquisition.

Leadership

The Time-Lapse Microscopy Facility is directed by Jeffrey L. Goldberg, MD, PhD

Location

The Time-Lapse Microscopy Facility is located on the 4th floor of the McKnight building, room 413E.

Equipment

  • Carl Zeiss compound beam-splitter binocular microscope (Axiovert 200m)
  • Zeiss MRm monochrome digital camera
  • DG4 light source
  • Dual-View Splitter
  • CoolSnap DS camera

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This microscope (Zeiss #4730129902) is equipped with a 360 degree rotary stage for alignment and also has epi-fluorescent attachments and a lamp housing made for multi-purpose microscopy including light, phase and immunofluorescence. The camera has 12-bit, 1300X1030 pixel resolution. It has the dual-view splitter, 2nd camera (coolsnap DS), DG4 light source for rapid excitation switching, and is capable of multi-fluorescence modalities such as quantitative calcium imaging and FRET, as well as software add-ons for cell/ object counting and mosaic image acquisition. It is equipped with a wide variety of quality objectives: 10x, 20x, 40x, 63x, and 100x plan APO. It also has filter cubes for imaging signals from multiple fluorophors including FITC, CFP, Rhodamine, YFP, Cy5, Indo1, and DAPI.